Phytochemical High Performance Thin Layer Chromatography based Estimation of Lawsone in Lawsonia inermis (Henna) obtained from Two Natural Habitats and Dye Products Collected from Local Market

The present study elucidates the preliminary phytochemical analysis and High Performance Thin Layer Chromatography (HPTLC) fingerprint analysis of lawsone extract of the leaves of Lawsonia inermis (henna) and evaluates their regions and products based quantitative estimations. The lawsone content has been evaluated quantitatively in two plants samples collected from Rewa and Bhopal and one commercial henna powder procured from open market of Bhopal city. Here we aimed to optimize high performance thin layer chromatography for the determination of lawsone. The chromatography development was carried out on the TLC silica gel 60 F254 aluminum plate and good resolution was achieved with toluene: ethyl acetate: formic acid (22:16:2) as mobile phase. Lawsone detected was carried out densitometrically at 254 nm by absorption mode and a linear regression coefficient was obtained with Y=133.527+13.470 × R=0.99092 ± 6.43%. The methods were applied are validated with respect to specificity, linearity, accuracy precision and robustness, and also find out the linear calibration come with respect to the concentration and regression coefficient. The developed method was validated as percentage relative standard deviation of peak areas was found to be which indicates the suitability of system for the further validation parameters. There was the presence of the peak at Rf 0.4 in the placebo, indicates the method is specific as none of the recipients interfered with the analytes of interest. The peak purity of Lawsone was achieved from 560-1865 Au.